Journal: Pharmaceuticals

Article Title: Fluorochrome Selection for Imaging Intraoperative Ovarian Cancer Probes

doi: 10.3390/ph15060668

Figure Lengend Snippet: Organ biodistribution and intraoperative NIR FLI; ( A ) “Ex vivo” NIR FLI 6 h after 500 µM injection of RR11 and 23 ( MSA14 ) was performed to assess the compound accumulation in tumors and organs. ( B ) Representative intraoperative near-infrared (NIR), color and pseudo-colored fluorescent signal merge images of one 23 ( MSA14 ) injected subcutaneously engrafted OVCAR-3 mouse in vivo and ex vivo.

Article Snippet: A second intraoperative compatible imaging system (FLARE ® , Curadel LLC, Nattick, MA, USA) was used to assess the clinical utility of 23 ( MSA14 ).

Techniques: Ex Vivo, Injection, In Vivo

Journal: Pharmaceuticals

Article Title: Fluorochrome Selection for Imaging Intraoperative Ovarian Cancer Probes

doi: 10.3390/ph15060668

Figure Lengend Snippet: Biodistribution of RR11 and 23 ( MSA14 ) in SKOV-3 s.c. models. RR11 ( A ) and 23 ( MSA14 ) ( B ) were intravenously injected into n = 2 and n = 4 mice, respectively, and imaged at 2, 6, and 24 h with a dose of 500 and 100 µM. The fluorescence signal in the tumors (*), liver (#), limb (†), spine (‡) and background in the flank muscle (yellow square), is presented in one representative mouse. All animals were euthanized when indicated and “ex vivo” NIR FLI was performed. ( C ) 23 (MSA14) injected SKOV-3 mice were additionally imaged with the intraoperative imaging system “in vivo” and “ex vivo”. NIR exposure times are indicated in brackets.

Article Snippet: A second intraoperative compatible imaging system (FLARE ® , Curadel LLC, Nattick, MA, USA) was used to assess the clinical utility of 23 ( MSA14 ).

Techniques: Injection, Fluorescence, Ex Vivo, Imaging, In Vivo

Journal: Cancer Medicine

Article Title: Trastuzumab‐based near‐infrared photoimmunotherapy in xenograft mouse of breast cancer

doi: 10.1002/cam4.5302

Figure Lengend Snippet: Evaluation of NIR‐PIT effects in BT‐474 tumor model. (A) The regimen of NIR‐PIT in vivo. Fluorescence images were obtained at each time point as indicated. (B) Time‐course imaging of in vivo Tra‐IR700 fluorescence in BT‐474 tumor‐bearing mice in response to NIR‐PIT. Tumors treated with NIR‐PIT showed decreased Tra‐IR700 fluorescence just after NIR light irradiation. Upper‐left panel uses same data as shown in Figure (right‐middle panel). (C) Intensity ratio of Tra‐IR700 fluorescence before and after NIR‐PIT treatment. The NIR‐PIT‐treated group showed significantly decreased Tra‐IR700 fluorescence compared with the Tra‐IR700 only group ( n = 3–10; Aspin–Welch's t ‐test; ** p < 0.05). (D) Histological images of resected BT‐474 tumor 1 day after NIR‐PIT. In the NIR‐PIT group, tumor cells showed diffuse cellular necrosis and micro‐hemorrhage. Black scale bars = 1 mm. White scale bars = 50 μm. (E) Quantification of damaged areas of tumor tissue by NIR‐PIT. The damaged areas of tumor tissue were scored by percentage (%) in histological images. In the NIR‐PIT group, the tumor tissue damage was significantly increased compared with the NIR‐PIT‐untreated group ( n = 5–10; Aspin–Welch's t ‐test: ** p < 0.05). (F) NADH‐TR‐stained images of BT‐474 tumor sections 1 day after NIR‐PIT. In the NIR‐PIT group, NADH staining was lacking. Black scale bars = 1 mm. White scale bars = 50 μm.

Article Snippet: Macroscopic images of mice were captured using an in‐house NIR fluorescence imaging system consisting of excitation light by LEDs at a peak of 660 nm (SMBB660‐1100‐02; Ushio Opto Semiconductors) and a monochrome camera (GS3‐U3‐15S5M‐C, Point Grey Research) equipped with a 692‐nm long‐pass filter (FF01‐692/LP‐25‐D; Semrock).

Techniques: In Vivo, Fluorescence, Imaging, Irradiation, Staining

Journal: Biomedical Optics Express

Article Title: High resolution combined molecular and structural optical imaging of colorectal cancer in a xenograft mouse model

doi: 10.1364/BOE.9.006186

Figure Lengend Snippet: a) PET-CT image of a mouse bearing LS174T colon cancer xenografts i.p. b) A wide-field NIR fluorescence image of the same mouse showing the accumulation of [89Zr]Zr-labetuzumab-IRDye800CW i.p. The greyscale range is [5.0–12]∙106 photons/s∙mm2. c) The large tumor visible in images (a) and (b) was excised and imaged again ex vivo with the wide-field NIR fluorescence imager. The color-scale range is [4.2–58]∙106 photons/s∙mm2. (i.e. fluorescence background and maximum value). d) The same excised tumor imaged with our custom NIRF scanner. The tumor was imaged from the opposite side with respect to the wide-field NIR fluorescence imager. The grayscale ranges from the background count rate to 0.8 times the maximum count rate of the image, in numbers: [1.8–21.6]∙106 photons/s. e) Zoomed in view of the area marked by the red square in (d) showing heterogeneity of the tumor. The connective tissue appears as white (low-fluorescence) areas. The greyscale range is [1.8–21.6]∙106 photons/s. All scale bars are 500 µm.

Article Snippet: Thereafter, mice were placed in a wide-field NIR fluorescence imager (In-Vivo Xtreme Imager, Bruker, Leiderdorp, The Netherlands) with excitation and emission filters of 760 nm and 830 nm, respectively for NIR fluorescence imaging for 5 sec. After imaging, the mice were sacrificed and all visible tumor lesions were taken out for ex vivo wide-field NIR fluorescence imaging.

Techniques: Positron Emission Tomography-Computed Tomography, Fluorescence, Ex Vivo